Presented by Adrienne DePorre, MD
Resident, Department of Pediatrics
- Antibodies to blood group antigens (anti-A/anti-B) are found in all preparations of IVIG as a result of pooling from O donors. Average antiblood titers are 1:16 for A and 1:8 for B. Those that developed hemolysis often had antiblood group titers of greater than 1:32. This combined with higher affinity antibodies can cause hemolysis.
- There is an increased risk with higher doses of IVIG in those who receive greater amounts of pathogenic antibodies.
- It is standard practice now to monitor the patient’s hemoglobin 24 to 48 hours after completing IVIG and one week after discharge, and to be concerned if the hemoglobin drops after 1 dose of IVIG.
- DAT negative autoimmune hemolytic anemia (AIHA) represents 3-10% of all AIHA, most commonly because RBC-bound IgA/IgM is not detectable by routine reagents, and because low affinity IgG is washed off the RBCs during the washing phase of the DAT.
- If strongly suspecting AIHA, it may be helpful to find a reference lab where special reagents (such as a LISS test or a direct polybrene test) are available.
Berard R, Whittemore B, Scuccimarri R. Hemolytic anemia following intravenous immunoglobulin therapy in patients treated for Kawasaki disease: a report of 4 cases. Pediatr Rheumatol Online J. 2012 Apr 16; 10(1):10. PMID: 22507284.
Garratty G, Leger RM, Hunt P, Co A. Serological investigation of a large series of direct antiglobulin test negative hemolytic anemias (SP313). Transfusion. 2004; 44 (Suppl 1): 121A-122A.
Wilson JR, Bhoopalam H, Fisher M. Hemolytic anemia associated with intravenous immunoglobulin. Muscle Nerve. 1997 Sep; 20(9):1142-5. PMID: 9270670.
Savage N, Georgi D, Kutlar A, Cook L. Coombs’-negative autoimmune hemolytic anemia in a patient with small lymphocytic lymphoma involving the bone marrow. LabMedicine 2008; 39 (8): 465-466.